WebMutation of the Candida albicans KEX2 gene results in defects in cell morphology and hyphal formation, while Candida glabrata kex2 mutants exhibit defects in cell surface integrity [14,54]. One identified substrate of C. albicans kexin is the endoplasmic reticulum α1,2-mannosidase, which is converted to a soluble form by cleavage [31]. WebHayes BM's publication in Antimicrob Agents Chemother.... and a KEX2 101 cleavage site at the 5' end and a NotI restriction endonuclease site and a stop codon at the 3' 102 end was purchased from for cloning into the pPIC9 vector. The STE13 protease 103 site between the ...
Zhu YS, et al. (1992) SGD
Webv Important Information Contents 6 μg of each of pPICZα A, B, and C vector in TE buffer, pH 8.0* (40 μl at 150 ng/μl) *TE buffer, pH 8.0: 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 Shipping/Storage The vectors are shipped on wet ice and should be stored at –20°C. Reference Sources The pPICZα A, B, and C vectors may be used with the Original … WebKex2 protease is the prototype for a family of proteases responsible for endoproteolytic cleavage at multi-basic motifs in the eukaryotic secretory pathway. Here we demonstrate that potassium ion can act as a modulator of Kex2 activity with an apparent affinity of ∼20 mm. Other monovalent cations (Li+, Na+, etc.) display similar effects, but affinities are all … scissor gesture
Production of the biotechnologically relevant AFP from Aspergillus ...
WebKexin ( EC 3.4.21.61) is a prohormone -processing protease, specifically a yeast serine peptidase, [1] found in the budding yeast ( S. cerevisiae ). It catalyzes the cleavage of … WebEffectors of biotrophic fungal plant pathogens Pamela H. P. GanA, Maryam RafiqiA, Adrienne R. HardhamA and Peter N. DoddsB,C APlant Science Division, Research School of Biology, College of Medicine, Biology and Environment, Australian National University, Canberra, ACT 2601, Australia. BCSIRO Plant Industry, GPO Box 1600, Canberra, ACT … Web1 sep. 1998 · The specificity of the yeast proprotein-processing Kex2 protease was examined in vivo by using a sensitive, quantitative assay. A truncated prepro-alpha-factor gene encoding an alpha-factor precursor with a single alpha-factor repeat was constructed with restriction sites for cassette mutagenesis flanking the single Kex2 cleavage site ( … scissor goddess 170